Pdgfra^{em1(rtTA)Xsun}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Pdgfra^{em1(rtTA)Xsun}
• Name: platelet derived growth factor receptor, alpha polypeptide; endonuclease-mediated mutation 1, Xin Sun
• MGI ID: MGI:6379301
• Synonyms: Pdgfra^rtTA
• Gene: Pdgfra Location: Chr5:75312953-75358876 bp, + strand Genetic Position: Chr5, 39.55 cM
• Alliance: Pdgfra^{em1(rtTA)Xsun} page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Transactivator)
• Mutation: Insertion
• Mutation details: CRISPR/Cas9 genome editing used a guide RNA (gRNA; 5'-GAGACAGTAATTGAGGATCCTGG-3'), a double-stranded donor plasmid (containing a splice acceptor (SA), reverse tetracycline-regulated transactivator (rtTA), and a trimerized simian virus 40 polyadenylation (3 pA) to introduce rtTA to the first intron) of the gene. The mutation is anticipated to create a null allele. (J:281590)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Pdgfra Mutation: 88 strains or lines available

References

• Original: J:281590 Li R, et al., Pdgfra marks a cellular lineage with distinct contributions to myofibroblasts in lung maturation and injury response. Elife. 2018 Sep 4;7:e36865
• All: 5 reference(s)