Timeless^em1Ljp
Endonuclease-mediated Allele Detail

Summary

• Symbol: Timeless^em1Ljp
• Name: timeless circadian clock 1; endonuclease-mediated mutation 1, Louis J Ptacek
• MGI ID: MGI:6369043
• Synonyms: Tim-R1078X
• Gene: Timeless Location: Chr10:128067934-128088810 bp, + strand Genetic Position: Chr10, 76.49 cM
• Alliance: Timeless^em1Ljp page

Mutation origin

• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Endonuclease-mediated (Humanized sequence)
• Mutation: Single point mutation
• Mutation details: Using CRISPR/Cas9 technology with sgRNAs and a 200-nt single-stranded oligodeoxynucleotides (ssODN), a mutation was introduced to change arginine codon 1078 to a stop codon (p.R1078*). This mutation mimics the p.R1081* found in familial advanced sleep phase (FASP) patients. (J:276566)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Timeless Mutation: 61 strains or lines available

References

• Original: J:276566 Kurien P, et al., TIMELESS mutation alters phase responsiveness and causes advanced sleep phase. Proc Natl Acad Sci U S A. 2019 Jun 11;116(24):12045-12053
• All: 1 reference(s)