Stk33^tm1.2Csll
Targeted Allele Detail

Summary

• Symbol: Stk33^tm1.2Csll
• Name: serine/threonine kinase 33; targeted mutation 1.2, Claudia Scholl
• MGI ID: MGI:6368790
• Synonyms: Stk33.KO
• Gene: Stk33 Location: Chr7:108878430-109038288 bp, - strand Genetic Position: Chr7, 57.21 cM
• Alliance: Stk33^tm1.2Csll page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:253666
• Parent Cell Line: v6.5 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A targeting vector was designed to replace the Stk33 locus between exon 6 and exon 8 with a cassette containing exon 7 of Stk33, a neomycin resistance gene flanked by FRT sites, and loxP sites surrounding exon 7 and the neomycin cassette. The mice that still contained the neomycin cassette were bred with CMV-Cre mice leading to germline deletion of Stk33 exon 7 together with the neomycin cassette creating the straight Stk33 knockout mouse. The deletion of exon 7 removes part of the Stk33 kinase domain and leads to a frameshift resulting in a premature stop codon at the second amino acid in exon 8. Immunohistochemistry and western blot confirmed absence of protein in testis, however shortened proten lucking 76 amino acids of the kinase domain including the active site coded by exon 7 and exon 8 were present in the lung and oviduct. (J:253666)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Stk33 Mutation: 29 strains or lines available

References

• Original: J:253666 Martins LR, et al., Stk33 is required for spermatid differentiation and male fertility in mice. Dev Biol. 2018 Jan 1;433(1):84-93
• All: 1 reference(s)