Cyp4x1^tm1.1Beld
Targeted Allele Detail

Summary

• Symbol: Cyp4x1^tm1.1Beld
• Name: cytochrome P450, family 4, subfamily x, polypeptide 1; targeted mutation 1.1, David Bell
• MGI ID: MGI:6368621
• Synonyms: Cyp4x1^-
• Gene: Cyp4x1 Location: Chr4:114963518-114991276 bp, - strand Genetic Position: Chr4, 52.76 cM
• Alliance: Cyp4x1^tm1.1Beld page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:254435
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: By homologous recombination in ES cells a single loxP site was inserted into intron 3 and FRT flanked PGK-neo cassette followed by loxP was inserted into intoron 4, resulting in Exon 4 flanked by loxP sites. Heterozygous Cyp4x1^tm1Beld mice are crossed with cre deleter to remove Exon 4 and PGK-neo cassette, resulting in frame-shift mutaion. (J:254435)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Cyp4x1 Mutation: 27 strains or lines available

References

• Original: J:254435 Kharkwal H, et al., Generation and phenotypic characterisation of a cytochrome P450 4x1 knockout mouse. PLoS One. 2017;12(12):e0187959
• All: 1 reference(s)