Utf1^em1Hoch
Endonuclease-mediated Allele Detail

Summary

• Symbol: Utf1^em1Hoch
• Name: undifferentiated embryonic cell transcription factor 1; endonuclease-mediated mutation 1, Konrad Hochedlinger
• MGI ID: MGI:6368147
• Synonyms: Utf1-GFP
• Gene: Utf1 Location: Chr7:139523769-139525025 bp, + strand Genetic Position: Chr7, 84.91 cM, cytoband F5
• Alliance: Utf1^em1Hoch page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:271499
• Parent Cell Line: v6.5 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Endonuclease-mediated (Reporter)
• Mutation: Intragenic deletion
• Mutation details: Plasmids encoding an sgRNA (5'(GACTGATAACAAAGCTTTAT-3'))are designed to introduce an E2A-GFP cassette in-frame in the final exon of the gene. (J:271499)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Utf1 Mutation: 19 strains or lines available

Notes

These V6.5 ES cells were previously targeted with doxycycline inducible Col1a1^{tm1(tetO-Pou5f1,-Klf4,-Sox2,-Myc)Hoch} and Gt(ROSA)26Sor^{tm1(rtTA*M2)Jae}.

References

• Original: J:271499 Schwarz BA, et al., Prospective Isolation of Poised iPSC Intermediates Reveals Principles of Cellular Reprogramming. Cell Stem Cell. 2018 Aug 2;23(2):289-305.e5
• All: 1 reference(s)