Celf4^{em1(icre/ERT2)Zyliu}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Celf4^{em1(icre/ERT2)Zyliu}
• Name: CUGBP, Elav-like family member 4; endonuclease-mediated mutation 1, Zhiyong Liu
• MGI ID: MGI:6368146
• Synonyms: Celf4-3xHA-P2A-iCreER-T2A-EGFP
• Gene: Celf4 Location: Chr18:25610689-25887214 bp, - strand Genetic Position: Chr18, 13.92 cM, cytoband B1
• Alliance: Celf4^{em1(icre/ERT2)Zyliu} page

Mutation origin

• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Endonuclease-mediated (Inducible, Recombinase, Reporter)
• Inducer: tamoxifen
• Mutation: Insertion
• Celf4^{em1(icre/ERT2)Zyliu} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Celf4 (MGI:1932407)

• Mutation details: Using CRISPR/Cas9 with single guide RNAs and a targeting vector, the following elements were inserted immediately upstream of the stop codon: a 3x hemagglutinin tag (HA) followed by sequence for self-cleaving oligopeptide P2A fused to a codon improved Cre recombinase and estrogen receptor 1 (human) fusion gene, and sequence for self-cleaving oligopeptide T2A fused to an enhanced green fluorescent protein sequence (EGFP) gene. This allele thus expresses the endogenous peptide, Cre and EGFP under the control of the endogenous promoter. (J:293995)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Celf4 (mouse) Summary of all recombinase alleles driven by Celf4. Alliance mouse Celf4 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Celf4 Mutation: 27 strains or lines available

References

• Original: J:293995 Li C, et al., Comprehensive transcriptome analysis of cochlear spiral ganglion neurons at multiple ages. Elife. 2020 Jan 8;9:e50491
• All: 1 reference(s)