Tg(CAG-cre)#Ben
Transgene Detail

Summary

• Symbol: Tg(CAG-cre)#Ben
• Name: transgene insertion, Rui Benedito
• MGI ID: MGI:6361145
• Synonyms: Tg(iSuRe-Cre)
• Transgene: Tg(CAG-cre)#Ben Location: unknown
• Alliance: Tg(CAG-cre)#Ben page

Transgene origin

• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Transgene description

• Transgene Type: Transgenic (Conditional ready, Recombinase, Reporter)
• Mutation: Insertion
• Tg(CAG-cre)#Ben expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  chicken	CAG

• Mutation details: The transgene contains four chicken -globin HS4 insulator sequences (two on each ends) flanking the ubiquitous and strong CAG promoter driving LoxP-flanked (floxed) DNA sequence which has the N-PhiM reporter gene encoding a noncytotoxicand non-fluorescent mutated PhiYFP protein with a nuclear localization signal and a transcription stop signal followed by bright membrane-localized fluorescent reporter (MbTomato) and cre gene separated by 2A cleavage site (T2A; mediates ribosomal skipping), then a WPRE sequence, an SV40 polyA signal. A Frt flanked PGK-neo cassette followed those sequences. After recombination/deletion of the floxed cassette by Cre or CreERT2, MbTomato and Cre protein are constitutively active and permanently expressed. Founder information and line numbers were not available. The pound symbol (#) is used when no line is specified and/or lines are pooled. (J:278452)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	CAG (chicken) Summary of all recombinase alleles driven by CAG.

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:278452 Fernandez-Chacon M, et al., iSuRe-Cre is a genetic tool to reliably induce and report Cre-dependent genetic modifications. Nat Commun. 2019 May 22;10(1):2262
• All: 2 reference(s)