Gt(ROSA)26Sor^{tm2(CAG-cas9*/ZNF10*)Gers}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm2(CAG-cas9*/ZNF10*)Gers}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 2, Charles Gersbach
• MGI ID: MGI:6360904
• Synonyms: Rosa26-LSL-dCas9-KRAB
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm2(CAG-cas9*/ZNF10*)Gers} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:342286
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Conditional ready, Endonuclease, Epitope tag)
• Mutation: Insertion
• Mutation details: The targeting vector is designed with (from 5' to 3') a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG), an FRT site, a loxP-flanked STOP cassette, a 3xFLAG epitope tag, an SV40 nuclear localization signal, a dCas9-KRAB sequence (dCas9-NLS-KRAB; see below), a WPRE, a bGH polyA signal and an attB-PGK-frt-Neo-polyA-attP cassette. This entire construct was inserted between exons 1 and 2 of the Gt(ROSA)26Sor locus. The dCas9-KRAB is composed of a human codon-optimized, nuclease-deficient Streptococcus pyogenes Cas9 protein synthetic DNA fused at its C-terminus to a nuclear localization signal and the repressive chromatin modifier domain mRNA from the Kruppel associated box (KRAB) domain of the human zinc-finger protein 10 gene (ZNF10). (J:342286)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 944 strains or lines available

References

• Original: J:342286 Gemberling MP, et al., Transgenic mice for in vivo epigenome editing with CRISPR-based systems. Nat Methods. 2021 Aug;18(8):965-974
• All: 1 reference(s)