Col1a1^{tm24(CAG-AKT*,-luc2)Nki}
Targeted Allele Detail

Summary

• Symbol: Col1a1^{tm24(CAG-AKT*,-luc2)Nki}
• Name: collagen, type I, alpha 1; targeted mutation 24, The Netherlands Cancer Institute
• MGI ID: MGI:6358646
• Synonyms: Col1a1^{invCAG-AktE17K-IRES-Luc}
• Gene: Col1a1 Location: Chr11:94827050-94843868 bp, + strand Genetic Position: Chr11, 59.01 cM, cytoband D
• Alliance: Col1a1^{tm24(CAG-AKT*,-luc2)Nki} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:233376
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Conditional ready, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Mutation details: The RMCE with the targeting vector introduced into the 3' UTR an inverted lox71/lox66-flanked CAG promoter driving ubiquitous expression of human cDNA with the amino acid substitution lysine for aspartic acid at position 17 (E17K; Open Biosystems 100067520), an IRES sequence and firefly luciferase (Luc2). (J:233376)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Col1a1 Mutation: 166 strains or lines available

Notes

Mutation created in ES cells containing Cdh1^tm1Jjon and Tg(Wap-cre)51Nki, which were subsequently bred out of the line.

References

• Original: J:233376 Annunziato S, et al., Modeling invasive lobular breast carcinoma by CRISPR/Cas9-mediated somatic genome editing of the mammary gland. Genes Dev. 2016 Jun 15;30(12):1470-80
• All: 2 reference(s)