Dlx6os1^tm2.1Jhdk
Targeted Allele Detail

Summary

• Symbol: Dlx6os1^tm2.1Jhdk
• Name: distal-less homeobox 6, opposite strand 1; targeted mutation 2.1, Jhumku D Kohtz
• MGI ID: MGI:6358615
• Synonyms: Evf1^TS
• Gene: Dlx6os1 Location: Chr6:6820543-6871627 bp, - strand Genetic Position: Chr6, 2.68 cM
• Alliance: Dlx6os1^tm2.1Jhdk page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:265948
• Parent Cell Line: W4 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Not Specified)
• Mutation: Insertion
• Mutation details: A STOP cassette (triple polyadenylation signal sequences) was inserted into exon 3, truncating Dlx6os1 splice variant Evf2. This generates an allele that doesn't express Evf1, but expresses truncated enhancer-containing form Evf2-5' that lacks the Evf2 3' end. The Evf2-5' transcript retains transcription across the Dlx5/Dlx6 ultra-conserved enhancer (UCE) Rr291 and retains Dlx6 antisense transcription. Expression of Dlx5 is increased. A loxP site flanked neomycin resistance gene cassette was removed by cre-mediated recombination. (J:265948)

Expression

In Mice Carrying this Mutation:

16 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Dlx6os1 Mutation: 3 strains or lines available

References

• Original: J:265948 Cajigas I, et al., The Evf2 Ultraconserved Enhancer lncRNA Functionally and Spatially Organizes Megabase Distant Genes in the Developing Forebrain. Mol Cell. 2018 Sep 20;71(6):956-972.e9
• All: 2 reference(s)