Tnr^{Tg(L1-EGFP)SN1Fhg}
Transgenic Allele Detail

Summary

• Symbol: Tnr^{Tg(L1-EGFP)SN1Fhg}
• Name: tenascin R; transgene insertion 1, Fred H Gage
• MGI ID: MGI:6358461
• Synonyms: 5'UTR-ORFeus, L1 transgene, SN1
• Gene: Tnr Location: Chr1:159351339-159759299 bp, + strand Genetic Position: Chr1, 69.03 cM, cytoband E2
• Alliance: Tnr^{Tg(L1-EGFP)SN1Fhg} page

Transgene origin

• Strain of Origin: C57BL/6J or (C57BL/6 x SJL)F1

Transgene description

• Transgene Type: Transgenic (Reporter)
• Mutation: Insertion
• Mutation details: The 8529 bp transgene contains an endogenous mouse long-interspersed nuclear element 1 (LINE-1 or L1) promoter, the codon-optimized mouse L1 ORF1 and ORF2 sequences, an EGFP-based retrotransposition indicator cassette and an SV40 polyA sequence. Founder line SN1 was found to have one L1 transgene copy (using qPCR) inserted into the first intron of the mouse tenascin R locus (Tnr) on chromosome 1. It is unknown whether the transgene affects Tnr function. Of note, construct contains some sequence from mCherry/dsRed, however, no expression is detected. (J:244330)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Tnr Mutation: 65 strains or lines available

References

• Original: J:244330 Newkirk SJ, et al., Intact piRNA pathway prevents L1 mobilization in male meiosis. Proc Natl Acad Sci U S A. 2017 Jul 11;114(28):E5635-E5644
• All: 4 reference(s)