Gt(ROSA)26Sor^{tm1(CAG-Vps4b*)1Hjug}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(CAG-Vps4b*)1Hjug}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Harald Junge
• MGI ID: MGI:6357993
• Synonyms: VPS4 EQ
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(CAG-Vps4b*)1Hjug} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:249954
• Parent Cell Line: EC7.1 (ES Cell)
• Strain of Origin: (C57BL/6J x 129X1/SvJ)F1

Mutation description

• Allele Type: Targeted (Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: A dominant-negative isoform of mouse Vps4b (Vps4b-EQ) cDNA with MycHis tag and bovine growth hormone polyadenylation sequence were placed under the control of a CAG promoter and lox-stop-lox sequence. VPS4b EQ has a modified E235Q in the encoded protein, which strongly inhibited signalling activity. (J:249954)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 944 strains or lines available

References

• Original: J:249954 Zhang C, et al., Norrin-induced Frizzled4 endocytosis and endo-lysosomal trafficking control retinal angiogenesis and barrier function. Nat Commun. 2017 Jul 04;8:16050
• All: 1 reference(s)