Bach2^tm2.1Kuro
Targeted Allele Detail

Summary

• Symbol: Bach2^tm2.1Kuro
• Name: BTB and CNC homology, basic leucine zipper transcription factor 2; targeted mutation 2.1, Tomohiro Kurosaki
• MGI ID: MGI:6357853
• Synonyms: Bach2^Flag
• Gene: Bach2 Location: Chr4:32238804-32586108 bp, + strand Genetic Position: Chr4, 14.02 cM, cytoband A4
• Alliance: Bach2^tm2.1Kuro page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:272661
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

• Allele Type: Targeted (Epitope tag)
• Mutation: Insertion
• Mutation details: The targeting construct containing a Frt-flanked Neo gene, a diphtheria toxin A (DTA), and a 3xFlag-tag was used for homologous recombination in ES cells to insert the flag tag after the first methionine of the Bach2 gene. Germline transmitted mice were bred with FLPe mice to remove the neo gene. (J:272661)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Bach2 Mutation: 44 strains or lines available

References

• Original: J:272661 Herndler-Brandstetter D, et al., KLRG1(+) Effector CD8(+) T Cells Lose KLRG1, Differentiate into All Memory T Cell Lineages, and Convey Enhanced Protective Immunity. Immunity. 2018 Apr 17;48(4):716-729.e8
• All: 2 reference(s)