Rbks^{tm1d(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Rbks^{tm1d(EUCOMM)Wtsi}
• Name: ribokinase; targeted mutation 1d, Wellcome Trust Sanger Institute
• MGI ID: MGI:6356625
• Synonyms: Rbks^delta2
• Gene: Rbks Location: Chr5:31781783-31854971 bp, - strand Genetic Position: Chr5, 17.27 cM
• Alliance: Rbks^{tm1d(EUCOMM)Wtsi} page
• IMPC: Rbks gene page

Mutation origin

• Mutant Cell Line: EPD0116_3_A10
• Germline Transmission: Earliest citation of germline transmission: J:236053
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 31830399 of Chromosome 5 upstream of the critical exon (exon 2) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon at position 31831256. Exon 2 is thus flanked by loxP sites. A null/knockout allele was created by flp and cre recombinase expression in mice carrying this allele to remove the lacZ sequence, neo selection cassette and exon 2. RT-PCR analysis confirmed deletion of exon 2 at the RNA level. (J:236053)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rbks Mutation: 70 strains or lines available

Notes

Cell line EPD0116_3_A10 was successfully used to make chimeric mice. Germline transmission was accomplished. J:236053

References

• Original: J:236053 Liu Y, et al., Ribose Accelerates Gut Motility and Suppresses Mouse Body Weight Gaining. Int J Biol Sci. 2016;12(6):701-9
• All: 1 reference(s)