Gramd1b^em1Pton
Endonuclease-mediated Allele Detail

Summary

• Symbol: Gramd1b^em1Pton
• Name: GRAM domain containing 1B; endonuclease-mediated mutation 1, Peter Tontonoz
• MGI ID: MGI:6356548
• Synonyms: Aster-B knockout, Gramd1b^-
• Gene: Gramd1b Location: Chr9:40204529-40442679 bp, - strand Genetic Position: Chr9, 21.42 cM, cytoband B
• Alliance: Gramd1b^em1Pton page

Mutation origin

• Strain of Origin: Not Specified

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Not Specified
• Mutation details: CRISPR/Cas9 mutagenesis induced a missense mutation in exon 7 that resulted in the introduction of a premature stop codon. Immunoblot analysis confirmed the absence of protein expression in adrenal lysates of homozygous mutant mice. (J:266462)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gramd1b Mutation: 120 strains or lines available

References

• Original: J:266462 Sandhu J, et al., Aster Proteins Facilitate Nonvesicular Plasma Membrane to ER Cholesterol Transport in Mammalian Cells. Cell. 2018 Oct 4;175(2):514-529.e20
• All: 1 reference(s)