Tmem98^{tm1b(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Tmem98^{tm1b(EUCOMM)Wtsi}
• Name: transmembrane protein 98; targeted mutation 1b, Wellcome Trust Sanger Institute
• MGI ID: MGI:6355415
• Gene: Tmem98 Location: Chr11:80701192-80712859 bp, + strand Genetic Position: Chr11, Syntenic
• Alliance: Tmem98^{tm1b(EUCOMM)Wtsi} page
• IMPC: Tmem98 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:277123
• Parent Cell Line: JM8A1.N3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 80705946 of Chromosome 11 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 80706751. The critical exon(s) is/are thus flanked by loxP sites.A null/knockout allele was created by cre recombinase expression in mice carrying the tm1a allele to remove the neo selection cassette and loxP-flanked critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:277123)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tmem98 Mutation: 25 strains or lines available

References

• Original: J:277123 Cross SH, et al., Missense Mutations in the Human Nanophthalmos Gene TMEM98 Cause Retinal Defects in the Mouse. Invest Ophthalmol Vis Sci. 2019 Jul 1;60(8):2875-2887
• All: 2 reference(s)