Thyn1^tm1Osb
Targeted Allele Detail

Summary

• Symbol: Thyn1^tm1Osb
• Name: thymocyte nuclear protein 1; targeted mutation 1, Research Institute for Microbial Diseases, Osaka University
• MGI ID: MGI:6343242
• Synonyms: Thy28^KI, Thyn1^{tm1a(EUCOMM)Osb}
• Gene: Thyn1 Location: Chr9:26911006-26918632 bp, + strand Genetic Position: Chr9, 12.06 cM, cytoband A4
• Alliance: Thyn1^tm1Osb page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:277335
• Parent Cell Line: EGR-G101 (ES Cell)
• Strain of Origin: C57BL/6NSlc-Tg(CAG-EGFP,Acr-EGFP)2Osb

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: This KO first allele (reporter-tagged insertion with conditional potential) was generated using EUCOMM vector PRPG00147_X_4_A07. The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exons. The critical exons 3-7 are thus flanked by loxP sites. For vector details, see Thyn1^{tm1a(EUCOMM)Hmgu}. (J:277335)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Thyn1 Mutation: 17 strains or lines available

References

• Original: J:277335 Kitaura F, et al., Normal B cell development and Pax5 expression in Thy28/ThyN1-deficient mice. PLoS One. 2019;14(7):e0220199
• All: 1 reference(s)