Ankrd31^em1Atot
Endonuclease-mediated Allele Detail

Summary

• Symbol: Ankrd31^em1Atot
• Name: ankyrin repeat domain 31; endonuclease-mediated mutation 1, Attila Toth
• MGI ID: MGI:6343192
• Synonyms: Ankrd31^-, Ankrd3^1mut1, mut1
• Gene: Ankrd31 Location: Chr13:96884797-97046302 bp, + strand Genetic Position: Chr13, Syntenic
• Alliance: Ankrd31^em1Atot page

Mutation origin

• Strain of Origin: Not Specified

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: CRIPSR/Cas9 genome editing created a 5 bp deletion as well as an insertion of four A residues in exon 6. This results in a frameshift after the 134th codon in the longest open reading frame, and is predicted to severely truncate the encoded protein. Immunoprecipitation-Western blot analysis of testis extracts and immunofluorescence in fixed gonadal samples confirmed that domains that are C-terminal to the frameshift position are not produced in homozygous mutant meiocytes. (J:276412)

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ankrd31 Mutation: 38 strains or lines available

References

• Original: J:276412 Papanikos F, et al., Mouse ANKRD31 Regulates Spatiotemporal Patterning of Meiotic Recombination Initiation and Ensures Recombination between X and Y Sex Chromosomes. Mol Cell. 2019 Jun 6;74(5):1069-1085.e11
• All: 1 reference(s)