Abhd4^tm1Crv
Targeted Allele Detail

Summary

• Symbol: Abhd4^tm1Crv
• Name: abhydrolase domain containing 4; targeted mutation 1, Benjamin F Cravatt
• MGI ID: MGI:6343190
• Synonyms: ABHD4^-
• Gene: Abhd4 Location: Chr14:54491586-54506626 bp, + strand Genetic Position: Chr14, 27.71 cM
• Alliance: Abhd4^tm1Crv page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:276423
• Parent Cell Line: CMTI-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 3 and 4 were replaced with a neomycin selection cassette. RT-PCR analysis confirmed loss of mRNA expression in brain and testis tissues from homozygous mutant mice. Lack of protein activity in mutant brains was confirmed by mass spectrometry (MS)-based activity-based protein profiling. (J:276423)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Abhd4 Mutation: 17 strains or lines available

References

• Original: J:276423 Lee HC, et al., ABHD4 regulates multiple classes of N-acyl phospholipids in the mammalian central nervous system. Biochemistry. 2015 Apr 21;54(15):2539-49
• All: 2 reference(s)