Pex5l^tm1.2Sieg
Targeted Allele Detail

Summary

• Symbol: Pex5l^tm1.2Sieg
• Name: peroxisomal biogenesis factor 5-like; targeted mutation 1.2, Steven A Siegelbaum
• MGI ID: MGI:6342269
• Synonyms: Pex5l^-
• Gene: Pex5l Location: Chr3:33003557-33197396 bp, - strand Genetic Position: Chr3, 15.94 cM, cytoband B
• Alliance: Pex5l^tm1.2Sieg page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:240397
• Parent Cell Line: KV1 (ES Cell)
• Strain of Origin: (C57BL/6J x 129S6/SvEvTac)F1

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: Using vector PRPGS00115_B_D04 from IMPC, the cassette inserted at position 33004820 of Chromosome 3 upstream of the critical exon 7 (Build GRCm38). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon at position 33004060. The critical exon(s) is/are thus flanked by loxP sites. Flp-mediated recombination removed the reporter and selection cassette. Cre-mediated recombination removed exon 8. (J:240397)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pex5l Mutation: 39 strains or lines available

References

• Original: J:240397 Srinivas KV, et al., The Dendrites of CA2 and CA1 Pyramidal Neurons Differentially Regulate Information Flow in the Cortico-Hippocampal Circuit. J Neurosci. 2017 Mar 22;37(12):3276-3293
• All: 1 reference(s)