Rr71^tm1.1Tyag
Targeted Allele Detail

Summary

• Symbol: Rr71^tm1.1Tyag
• Name: regulatory region 71; targeted mutation 1.1, Takeshi Yagi
• MGI ID: MGI:6336159
• Synonyms: HS5-1deltaNeo
• Gene: Rr71 Location: unknown Genetic Position: Chr18, Syntenic
• Alliance: Rr71^tm1.1Tyag page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:277249
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: Mouse BAC RP23-318M13 was used as the basis of the targeting vector. A loxP site was inserted downstream of the sequence containing DNase1 hypersensitivity regions HS1-HS5 (HS5-1). A loxP site flanked neomycin resistance gene cassette was inserted upstream and subsequently removed through in-vitro cre-mediated recombination, leaving a single loxP site upstream of the region. Next, an FRT site flanked neo cassette was inserted upstream of the 5' loxP site. This neo cassette was removed through subsequent flp-mediated recombination. The regulatory region is located downstream of the Pcdha cluster and regulates expression of its genes. (J:277249)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rr71 Mutation: 0 strains or lines available

References

• Original: J:277249 Yokota S, et al., Identification of the cluster control region for the protocadherin-beta genes located beyond the protocadherin-gamma cluster. J Biol Chem. 2011 Sep 9;286(36):31885-95
• All: 1 reference(s)