Tg(ACTA1-Ppargc1a_ib)AOse
Transgene Detail

Summary

• Symbol: Tg(ACTA1-Ppargc1a_ib)AOse
• Name: transgene insertion A, Osamu Ezaki
• MGI ID: MGI:6324957
• Transgene: Tg(ACTA1-Ppargc1a_ib)AOse Location: unknown
• Alliance: Tg(ACTA1-Ppargc1a_ib)AOse page

Transgene origin

• Strain of Origin: (C57BL/6 x DBA/1)F1

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence, Modified isoform(s))
• Mutation: Insertion
• Tg(ACTA1-Ppargc1a_ib)AOse expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Ppargc1a (MGI:1342774)	Human promoter and mouse isoform b cDNA

• Mutation details: The transgene contains the following elements: a 2.2 kb DNA fragment containing the promoter, exon 1 and (part of) intron 1 of the human skeletal muscle actin alpha 1 gene, the b isoform cDNA of the mouse peroxisome proliferative activated receptor gamma coactivator 1 alpha gene, and bovine growth hormone poly(A) signal sequences. The b splice variant incorporates a shorter version of alternative 5' exon 1b, coding for a longer alternative N-terminus of the peptide. Two lines (A and B), with different transgene expression levels, were established. (J:182259)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:182259 Tadaishi M, et al., Skeletal muscle-specific expression of PGC-1alpha-b, an exercise-responsive isoform, increases exercise capacity and peak oxygen uptake. PLoS One. 2011;6(12):e28290
• All: 1 reference(s)