Tg(Cdh5-COP4*L132C,-lacZ)B28-2Mik
Transgene Detail
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Symbol: |
Tg(Cdh5-COP4*L132C,-lacZ)B28-2Mik |
Name: |
transgene insertion B28-2, Michael I Kotlikoff |
MGI ID: |
MGI:6324920 |
Synonyms: |
B28-Cdh5BAC-CatCh2_IRES_lacZ |
Transgene: |
Tg(Cdh5-COP4*L132C,-lacZ)B28-2Mik Location: unknown
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Alliance: |
Tg(Cdh5-COP4*L132C,-lacZ)B28-2Mik page
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Strain of Origin: |
C57BL/6J x SJL/J
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Transgene Type: |
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Transgenic (Reporter) |
Mutation: |
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Insertion
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Mutation details: Cdh5-CatCh-IRES-lacZ transgenic mice (also called Cdh5-CatCh-IRES-lacZ) were designed in the laboratory of Dr. Michael I. Kotlikoff (Cornell University) as part of Cornell/National Heart Lung Blood Resource for Optogenetic Mouse Signaling (CHROMus). The ~200 kbp C57BL/6J mouse bacterial artificial chromosome (BAC) RP23-453P1 was obtained; containing the entire cadherin 5 locus (Cdh5) as well as ~124 kbp of 5' flanking sequences (including the complete Gm32728, Gm39236 and Gm8748 predicted gene loci) and ~33 kbp of 3' flanking sequences (including a 5' portion of the Bean1 locus). Using homologous recombination/BAC recombineering, a 5640 bp CatCh-IRES-lacZ-pA construct (CatCh, internal ribosome entry site, β-galactosidase and an SV40 polyadenylation signal) was inserted into the ATG start site of the BAC Cdh5 gene (replacing the initiation codon of Cdh5 in exon 2). No other loc
i on the BAC were altered. The 11.6 kbp BAC vector (pBACe3.6) contained a chloramphenicol selection cassette.
(J:314125)
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 1 strain available
Cell Lines: 0 lines available
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Original: |
J:314125 Lee FK, et al., Genetically engineered mice for combinatorial cardiovascular optobiology. Elife. 2021 Oct 29;10:e67858 |
All: |
1 reference(s) |
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