Gt(ROSA)26Sor^{tm12(CAG-Venus*,-TagRFP*)Rkuhn}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm12(CAG-Venus*,-TagRFP*)Rkuhn}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 12, Ralf Kuhn
• MGI ID: MGI:6324910
• Synonyms: Gt(ROSA)26Sor^{tm1(CAG-Venus*,TagRFP*)Rkuhn}
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm12(CAG-Venus*,-TagRFP*)Rkuhn} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:101977
• Parent Cell Line: IDG3.2 (ES Cell)
• Strain of Origin: (C57BL/6J x 129S6/SvEvTac)F1

Mutation description

• Allele Type: Targeted (Reporter)
• Mutation: Insertion
• Mutation details: The R26-GFP_KI-TLR2 ("traffic light reporter") targeting vector containing Venus*-P2A-TagRFP-bovine hGH polyA, in a reading frame that is shifted by 2 bp (+3), under the control of a CAG promoter, and with an upstream PGK-neo cassette, two FRT sites and 2 recombined attP/B sites, was inserted into the Gt(ROSA)26Sor locus. The non-functional Venus in the TLR vector has codons 117-152 replaced with 52bp of mouse Gt(ROSA)26Sor and 56bp of mouse Rab38 (RAB38, member RAS oncogene family sequence). (J:101977, J:341573)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 944 strains or lines available

References

• Original: J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017
• All: 2 reference(s)