Mafg^tm1.1Jeng
Targeted Allele Detail

Summary

• Symbol: Mafg^tm1.1Jeng
• Name: v-maf musculoaponeurotic fibrosarcoma oncogene family, protein G (avian); targeted mutation 1.1, James Douglas Engel
• MGI ID: MGI:6324507
• Gene: Mafg Location: Chr11:120515943-120524426 bp, - strand Genetic Position: Chr11, 84.35 cM
• Alliance: Mafg^tm1.1Jeng page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:183645
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The entire coding region was replaced by a cassette containing lacZ and floxed tk-neo genes. The lacZ gene was situated immediately downstream of the translational start codon. RNAse protection assays were utilized to confirm the absence of transcript in homozygous mutant mice. Expression of beta-galactosidase was driven by the endogenous promoter. The loxP-flanked neomycin selection cassete was removed via cre-mediated recombination. (J:78982)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Mafg Mutation: 38 strains or lines available

References

• Original: J:183645 Yamazaki H, et al., Embryonic lethality and fetal liver apoptosis in mice lacking all three small Maf proteins. Mol Cell Biol. 2012 Feb;32(4):808-16
• All: 3 reference(s)