Nanos3^tm3Ysa
Targeted Allele Detail

Summary

• Symbol: Nanos3^tm3Ysa
• Name: nanos C2HC-type zinc finger 3; targeted mutation 3, Yumiko Saga
• MGI ID: MGI:6324490
• Synonyms: Nanos3-LpA(neo), Nanos3^tm4(lacZ)Ysa
• Gene: Nanos3 Location: Chr8:84900359-84911396 bp, - strand Genetic Position: Chr8, 40.45 cM
• Alliance: Nanos3^tm3Ysa page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:154912
• Parent Cell Line: TT2 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: A lacZ reporter gene was inserted at the translation initiation site. A loxP-flanked neomycin resistance cassette was inserted downstream of the reporter gene. Immunohistochemistry confirmed the absence of endogenous protein expression in the primordial germ cells. (J:154912)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nanos3 Mutation: 28 strains or lines available

References

• Original: J:154912 Suzuki H, et al., The heterogeneity of spermatogonia is revealed by their topology and expression of marker proteins including the germ cell-specific proteins Nanos2 and Nanos3. Dev Biol. 2009 Dec 15;336(2):222-31
• All: 1 reference(s)