Cetn3^{tm1d(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Cetn3^{tm1d(EUCOMM)Wtsi}
• Name: centrin 3; targeted mutation 1d, Wellcome Trust Sanger Institute
• MGI ID: MGI:6324197
• Synonyms: Cetn3^-
• Gene: Cetn3 Location: Chr13:81931196-81945274 bp, + strand Genetic Position: Chr13, 43.68 cM, cytoband C3
• Alliance: Cetn3^{tm1d(EUCOMM)Wtsi} page
• IMPC: Cetn3 gene page

Mutation origin

• Mutant Cell Lines: EPD0630_2_B09, EPD0630_2_E12
• Germline Transmission: Earliest citation of germline transmission: J:275959
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 81934502 of Chromosome 13 upstream of the critical exon 3 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 3 at position 81935301. Exon 3 is thus flanked by loxP sites. A null/knockout allele was created by flp and cre recombinase expression in mice carrying this allele to remove the lacZ sequence, neo selection cassette and the loxP-flanked critical exon. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. In J:275959, immunohistochemistry of retina cryosections confirmed the absence of protein in the connecting cilium and basal bodies of homozygous mutant retinal photoreceptors, indicating that this is a null allele. (J:275959)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cetn3 Mutation: 13 strains or lines available

Notes

Cell lines EPD0630_2_E12 and_ EPD0630_2_B09 were successfully used to make chimeric mice. Germline transmission was accomplished for both lines. J:275959

References

• Original: J:275959 Ying G, et al., Deletion of both centrin 2 (CETN2) and CETN3 destabilizes the distal connecting cilium of mouse photoreceptors. J Biol Chem. 2019 Mar 15;294(11):3957-3973
• All: 1 reference(s)