Col1a1^{tm19(CAG-cas9,-luc2)Nki}
Targeted Allele Detail

Summary

• Symbol: Col1a1^{tm19(CAG-cas9,-luc2)Nki}
• Name: collagen, type I, alpha 1; targeted mutation 19, The Netherlands Cancer Institute
• MGI ID: MGI:6316349
• Synonyms: Col1a1^{invCAG-Cas9-IRES-Luc}
• Gene: Col1a1 Location: Chr11:94827050-94843868 bp, + strand Genetic Position: Chr11, 59.01 cM, cytoband D
• Alliance: Col1a1^{tm19(CAG-cas9,-luc2)Nki} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:276500
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Conditional ready, Endonuclease, Reporter)
• Mutation: Insertion
• Mutation details: RMCE introduced into the 3' UTR an FRT-flanked cassette containing an inverted lox71/lox66-flanked CAG promoter, full-length cas9 sequence, IRES sequence and firefly luciferase reporter gene (fLuc2). (J:276500)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Col1a1 Mutation: 166 strains or lines available

Notes

Mutation created in ES cells containing Cdh1^tm1Jjon and Tg(Wap-cre)51Nki, which were subsequently bred out of the line.

References

• Original: J:276500 Annunziato S, et al., Comparative oncogenomics identifies combinations of driver genes and drug targets in BRCA1-mutated breast cancer. Nat Commun. 2019 Jan 23;10(1):397
• All: 4 reference(s)