Nxph4^em1Hzo
Endonuclease-mediated Allele Detail

Summary

• Symbol: Nxph4^em1Hzo
• Name: neurexophilin 4; endonuclease-mediated mutation 1, Huda Y Zoghbi
• MGI ID: MGI:6307097
• Synonyms: Nxph4^FLAG
• Gene: Nxph4 Location: Chr10:127361342-127370428 bp, - strand Genetic Position: Chr10, 74.51 cM
• Alliance: Nxph4^em1Hzo page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Epitope tag)
• Mutation: Insertion
• Mutation details: CRISPR/Cas9 genome editing is used to insert a 3xFLAG tag sequence at the end of the third domain of the gene (after 5'-CTCCAAGCGTGTGGAGTTC). The PAM site (AGG) was also mutated to AGA to avoid cleaving by Cas9. (J:278920)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Nxph4 Mutation: 24 strains or lines available

References

• Original: J:278920 Meng X, et al., Neurexophilin4 is a selectively expressed alpha-neurexin ligand that modulates specific cerebellar synapses and motor functions. Elife. 2019 Sep 16;8:e46773
• All: 1 reference(s)