Cers5^em1Btlr
Endonuclease-mediated Allele Detail

Summary

• Symbol: Cers5^em1Btlr
• Name: ceramide synthase 5; endonuclease-mediated mutation 1, Bruce Beutler
• MGI ID: MGI:6303830
• Gene: Cers5 Location: Chr15:99633473-99670396 bp, - strand Genetic Position: Chr15, 56.13 cM
• Alliance: Cers5^em1Btlr page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: The CRISPR/Cas9 system and sgRNA 5'-TGTTACTAAGGTAAGTGAGC-3' was used to generate the mice. The CRISPR/Cas9 system generated a 19-base pair deletion of CATCCGGCTCACTTACCTT at g.21298_21278 (NC_000081.6). The mutation is predicted to destroy the exon 2 donor splice site, resulting in the use of an adjacent cryptic site. The resulting transcript would have a 48-bp insertion causing an in-frame insertion of 16 amino acids beginning after amino acid 101 of the protein. (J:274568)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cers5 Mutation: 33 strains or lines available

References

• Original: J:274568 Choi JH, et al., LMBR1L regulates lymphopoiesis through Wnt/beta-catenin signaling. Science. 2019 May 10;364(6440)
• All: 1 reference(s)