Gt(ROSA)26Sor^{tm1(CAG-EGFP*)Thm}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(CAG-EGFP*)Thm}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Thomas Misgeld
• MGI ID: MGI:6296801
• Synonyms: Gt(ROSA)26Sor^{tm1(CAG-EGFP/Synj2bp)Thm}, MitoTag
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(CAG-EGFP*)Thm} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:281635
• Parent Cell Line: IDG3.2 (ES Cell)
• Strain of Origin: (C57BL/6J x 129S6/SvEvTac)F1

Mutation description

• Allele Type: Targeted (Reporter)
• Mutation: Insertion
• Mutation details: phiC32-mediated RMCE in ES cells containing PGK promoter::attP::hygro-pA::attP introduced into the loci a construct containing (from 5' to 3') an attB site, a neomycin resistance cassette (with polyA region), a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG), a loxP-flanked STOP cassette (LSL; puromycin resistance gene followed by a triple polyA region), a cDNA sequence composed of an enhanced green fluorescent protein sequence (EGFP) fused at its C-terminal to 37 C-terminal amino acids from the rat Omp25 protein (Synj2bp; aa109-145) which encodes a transmembrane domain for localization to the outer mitochondrial membrane, a bGH polyA region, and an attB site. (J:281635)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1032 strains or lines available

References

• Original: J:281635 Fecher C, et al., Cell-type-specific profiling of brain mitochondria reveals functional and molecular diversity. Nat Neurosci. 2019 Oct;22(10):1731-1742
• All: 5 reference(s)