Trem2^em3Bwef
Endonuclease-mediated Allele Detail

Summary

• Symbol: Trem2^em3Bwef
• Name: triggering receptor expressed on myeloid cells 2; endonuclease-mediated mutation 3, Benedikt Wefers
• MGI ID: MGI:6295472
• Synonyms: Trem2^{em3(H157Y)Bwef}, Trem2(H157Y), Trem2 p.H157Y
• Gene: Trem2 Location: Chr17:48653429-48659304 bp, + strand Genetic Position: Chr17, 23.99 cM, cytoband C
• Alliance: Trem2^em3Bwef page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Not Specified)
• Mutation: Single point mutation
• Mutation details: CRISPR/Cas9-mediated homologous recombination using a synthetic oligodeoxynucleotide created a nucleotide substitution (C to T) in exon 3 of Trem2 that results in the amino acid substitution H157Y. Two additional silent nucleotide exchanges within the protospacer/PAM region created an ApaLI restriction site for genotyping purposes.

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Trem2 Mutation: 65 strains or lines available

References

• Original: J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017
• All: 1 reference(s)