Dbh^tm2.2Pjen
Targeted Allele Detail

Summary

• Symbol: Dbh^tm2.2Pjen
• Name: dopamine beta hydroxylase; targeted mutation 2.2, Patricia Jensen
• MGI ID: MGI:6295470
• Synonyms: Dbh^flx, Dbh^hypo
• Gene: Dbh Location: Chr2:27055519-27073216 bp, + strand Genetic Position: Chr2, 19.29 cM
• Alliance: Dbh^tm2.2Pjen page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:341391
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Conditional ready, Hypomorph, Reporter)
• Mutation: Insertion
• Mutation details: A loxP site and a rox site flanked hygromycin/zeocin resistance gene cassette were inserted into intron 1. Sequence coding for a 2A peptide, tdTomato cDNA, SV40 poly(A) signal sequences, an FRT site flanked flanked neomycin resistance gene cassette, a second loxP site, a splice acceptor, EGFP cDNA, and rabbit beta-globin poly(A) signal sequences were inserted into exon 12 immediately before the stop codon. Subsequent Dre- and Flp-mediated recombination excised the rox- and FRT-flanked antibiotic resistance cassettes. The intron 1 loxP site insertion disrupts enhancer Rr60779, leading to reduced transcription (78-57%) of the gene in the brain. (J:341391)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dbh Mutation: 17 strains or lines available

References

• Original: J:341391 Wilson LR, et al., Partial or complete loss of norepinephrine differentially alters contextual fear and catecholamine release dynamics in hippocampal CA1. Biol Psychiatry Glob Open Sci. 2023;4(1):51-60
• All: 1 reference(s)