Kcnma1^em1Geh
Endonuclease-mediated Allele Detail

Summary

• Symbol: Kcnma1^em1Geh
• Name: potassium large conductance calcium-activated channel, subfamily M, alpha member 1; endonuclease-mediated mutation 1, Gregg E Homanics
• MGI ID: MGI:6294747
• Synonyms: FAP-Bkalpha
• Gene: Kcnma1 Location: Chr14:23342356-24055173 bp, - strand Genetic Position: Chr14, 12.92 cM
• Alliance: Kcnma1^em1Geh page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Epitope tag, Humanized sequence)
• Mutations: Insertion, Intragenic deletion
• Mutation details: CRISPR/Cas9 genome editing is used to replace 195 bp of exon 1 (including the first start codon) with an 855 bp insertion containing Igkappa signal sequence, an N-terminal HA epitope and an improved fluorogen activating protein sequence (L5** FAP) - such that the insertion is fused immediately 5' of the Kcnma1 downstream start codon. The L5** FAP sequence, originally derived from the human IGLV7-46 germline gamma allele (which appears to have undergone immune somatic hypermutation), was further engineered to have mutations conferring improved spectral and thermodynamic properties (L89S for increased quantum yield and E50D for greater MG affinity), as well as to function as a dimer. (J:273623)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Kcnma1 Mutation: 101 strains or lines available

References

• Original: J:273623 Pratt CP, et al., Tagging of Endogenous BK Channels with a Fluorogen-Activating Peptide Reveals beta4-Mediated Control of Channel Clustering in Cerebellum. Front Cell Neurosci. 2017;11:337
• All: 1 reference(s)