Tg(Myh6-GCaMP8)B4-10Mik
Transgene Detail

Summary

• Symbol: Tg(Myh6-GCaMP8)B4-10Mik
• Name: transgene insertion B4-10, Michael I Kotlikoff
• MGI ID: MGI:6294066
• Synonyms: aMHC-GCaMP8, B4-Myh6-GCaMP8
• Transgene: Tg(Myh6-GCaMP8)B4-10Mik Location: unknown
• Alliance: Tg(Myh6-GCaMP8)B4-10Mik page

Transgene origin

• Strain of Origin: FVB/N X B6(Cg)-Tyr^c-2J/J

Transgene description

• Transgene Type: Transgenic (Reporter)
• Mutation: Insertion
• Mutation details: The transgene was designed with (from 5' to 3') a ~5.5 kbp mouse Myh6 minimal promoter sequence (including the 3' most exon of betaMHC, the first three exons of alphaMHC [up to the start codon] as well as intervening sequences) and a ~1950 bp GCaMP8-hGHpA construct. GCaMP8 is composed of (from N-term to C-term) a polyHis plasmid leader sequence essential for thermal stability (RSET with the ΔH and ΔR2 mutations), a 20 residue peptide of chicken smooth muscle myosin light chain kinase (M13; target peptide for a Ca2+-bound CaM), a circularly permutated EGFP (cpEGFP; aa 149-238 followed by aa 1-144 [with mutations listed below]), a rat calmodulin DNA fragment (CaM; aa 2-148). Founder line B4-10 was identified with high sensor expression in cardiomyocytes. (J:314125)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:314125 Lee FK, et al., Genetically engineered mice for combinatorial cardiovascular optobiology. Elife. 2021 Oct 29;10:e67858
• All: 2 reference(s)