Optn^{tm1d(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Optn^{tm1d(EUCOMM)Wtsi}
• Name: optineurin; targeted mutation 1d, Wellcome Trust Sanger Institute
• MGI ID: MGI:6287658
• Synonyms: Optn null
• Gene: Optn Location: Chr2:5025453-5068862 bp, - strand Genetic Position: Chr2, 3.15 cM
• Alliance: Optn^{tm1d(EUCOMM)Wtsi} page
• IMPC: Optn gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:273195
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 5057375 of Chromosome 2 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 5058587. The critical exon(s) is/are thus flanked by loxP sites. A null/knockout allele was created by flp and cre recombinase expression in mice carrying this allele to remove the lacZ sequence, neo selection cassette and the loxP-flanked critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:273195)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Optn Mutation: 51 strains or lines available

References

• Original: J:234166 Ito Y, et al., RIPK1 mediates axonal degeneration by promoting inflammation and necroptosis in ALS. Science. 2016 Aug 5;353(6299):603-8
• All: 3 reference(s)