Il7^{tm1b(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Il7^{tm1b(EUCOMM)Wtsi}
• Name: interleukin 7; targeted mutation 1b, Wellcome Trust Sanger Institute
• MGI ID: MGI:6286242
• Synonyms: Il7^LacZKO
• Gene: Il7 Location: Chr3:7637088-7678820 bp, - strand Genetic Position: Chr3, 2.02 cM
• Alliance: Il7^{tm1b(EUCOMM)Wtsi} page
• IMPC: Il7 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:261603
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 7640807 of Chromosome 3 upstream of exon 3 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 4 at position 7642754. Cre-mediated recombination removed the promoter-driven neomycin selection cassette and exons 3 and 4 leaving behind the inserted lacZ reporter sequence. (J:261603)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Il7 Mutation: 33 strains or lines available

References

• Original: J:261603 Yang J, et al., IL-7-dependent maintenance of ILC3s is required for normal entry of lymphocytes into lymph nodes. J Exp Med. 2018 Apr 2;215(4):1069-1077
• All: 1 reference(s)