Kcnf1^em1(IMPC)H
Endonuclease-mediated Allele Detail

Summary

• Symbol: Kcnf1^em1(IMPC)H
• Name: potassium voltage-gated channel, subfamily F, member 1; endonuclease-mediated mutation 1, Harwell
• MGI ID: MGI:6283400
• Gene: Kcnf1 Location: Chr12:17222101-17226551 bp, - strand Genetic Position: Chr12, 8.04 cM
• Alliance: Kcnf1^em1(IMPC)H page
• IMPC: Kcnf1 gene page

Mutation origin

• Strain of Origin: C57BL/6NTac
• Project Collection: IMPC

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: This allele was generated at Medical Research Council Harwell using Cas9 and guides with spacer sequences ACTTGAACGCGTCTGGGTCC, AGGTCGCTGCGAGAACCCCC, CGGGGGTTCTCGCAGCGACC, and CTGTGCGACGATTACGATCC that targeted exon(s) ENSMUSE00000898935.3. This resulted in deletion(s) of 15 bp (location: Chr12:17225958-17225972; GRCm39), 1046 bp (location: Chr12:17224856-17225901; GRCm39). This description was generated automatically. Additional molecular information including FASTA sequence support for this allele can be found here: (IMPC Gene page) and viewed in a genome context here: (IMPC Genome Browser). (J:384794)

• Inheritance: Not Specified

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 3 strains available Cell Lines: 0 lines available
• Carrying any Kcnf1 Mutation: 25 strains or lines available

References

• Original: J:265051 MGI and IMPC, MGI Load of Endonuclease-Mediated Alleles (CRISPR) from the International Mouse Phenotyping Consortium (IMPC). Database Release. 2018-2023
• All: 4 reference(s)