Cep164^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Cep164^{tm1c(EUCOMM)Wtsi}
• Name: centrosomal protein 164; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6281982
• Synonyms: CEP164^fl
• Gene: Cep164 Location: Chr9:45678244-45739984 bp, - strand Genetic Position: Chr9, 25.1 cM
• Alliance: Cep164^{tm1c(EUCOMM)Wtsi} page
• IMPC: Cep164 gene page

Mutation origin

• Mutant Cell Lines: EPD0342_5_A03, EPD0342_5_B01
• Germline Transmission: Earliest citation of germline transmission: J:255142
• Parent Cell Line: JM8A1.N3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 45720772 of Chromosome 9 upstream of exon 4 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 4 at position 45721754. Flp-mediated recombination removed the FRT-flanked lacZ and neo cassette leaving exon 4 floxed. (J:255142)

Expression

In Structures Affected by this Mutation:

12 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cep164 Mutation: 40 strains or lines available

References

• Original: J:255142 Siller SS, et al., Conditional knockout mice for the distal appendage protein CEP164 reveal its essential roles in airway multiciliated cell differentiation. PLoS Genet. 2017 Dec;13(12):e1007128
• All: 4 reference(s)