Tg(Acta2-RHO/ADRA1,-lacZ)B6-1Mik
Transgene Detail

Summary

• Symbol: Tg(Acta2-RHO/ADRA1,-lacZ)B6-1Mik
• Name: transgene insertion B6-1, Michael I Kotlikoff
• MGI ID: MGI:6281614
• Synonyms: Acta2-Opto alpha;1AR-IRES-lacZ, B6-Acta2^BAC-Optoa1AR_IRES_lacZ
• Transgene: Tg(Acta2-RHO/ADRA1,-lacZ)B6-1Mik Location: unknown
• Alliance: Tg(Acta2-RHO/ADRA1,-lacZ)B6-1Mik page

Transgene origin

• Strain of Origin: FVB/N X B6(Cg)-Tyr^c-2J/J

Transgene description

• Transgene Type: Transgenic (Humanized sequence, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Mutation details: The transgenic construct is made using a C57BL/6J mouse bacterial artificial chromosome (BAC) RP23-370F21 containing the entire actin alpha 2 locus (Acta2) as well as ~92 kbp of 5' flanking sequences (including the complete Fas locus) and ~68 kbp of 3' flanking sequences (including the complete Stambpl1 locus). Using homologous recombination/BAC recombineering, a 6170 bp Opto alpha;1AR-IRES-lacZ-pA construct is inserted into the ATG start site of the BAC Acta2 gene (replacing the initiation codon in exon 2). No other loci on the BAC are altered. The 11.6 kbp BAC vector (pBACe3.6) contains a chloramphenicol selection cassette. The Opto alpha 1AR fusion protein is a Gt-coupled bovine rhodopsin (RHO) sequence modified to have its intracellular regions (including carboxy-terminal domains) replaced with those from the Gq-coupled human ADRA1A. Founder line B6-1 has high sensor expression in purified smooth muscle. (J:314125)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:314125 Lee FK, et al., Genetically engineered mice for combinatorial cardiovascular optobiology. Elife. 2021 Oct 29;10:e67858
• All: 1 reference(s)