Rab10^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Rab10^{tm1c(KOMP)Wtsi}
• Name: RAB10, member RAS oncogene family; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6281610
• Synonyms: Rab10^fl
• Gene: Rab10 Location: Chr12:3297428-3359969 bp, - strand Genetic Position: Chr12, 1.71 cM
• Alliance: Rab10^{tm1c(KOMP)Wtsi} page
• IMPC: Rab10 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:249843
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 3314374 of Chromosome 12 upstream of the critical exon 1 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon at position 3315039. The critical exon 1 is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:249843)

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rab10 Mutation: 25 strains or lines available

References

• Original: J:249843 Vazirani RP, et al., Disruption of Adipose Rab10-Dependent Insulin Signaling Causes Hepatic Insulin Resistance. Diabetes. 2016 Jun;65(6):1577-89
• All: 2 reference(s)