Pgf^tm1.1Sdf
Targeted Allele Detail

Summary

• Symbol: Pgf^tm1.1Sdf
• Name: placental growth factor; targeted mutation 1.1, Sandro De Falco
• MGI ID: MGI:6281343
• Synonyms: PIGF-DE-Ki
• Gene: Pgf Location: Chr12:85213413-85224087 bp, - strand Genetic Position: Chr12, 39.58 cM, cytoband D
• Alliance: Pgf^tm1.1Sdf page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:271257
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutation: Nucleotide substitutions
• Mutation details: Two point mutations that result in an aspartate to alanine substitution at amino acid 67 and a glutamate to alanine substitution at amino acid 68 were introduced in exon 3 and a loxP flanked PGK-neomycin cassette was inserted upstream of exon 3. Cre-mediated recombination removed the neomycin cassette. These two mutations generate the PGF-DE variant seen in humans that results in the loss of binding and activation of FLT1 (VEGFR-1) but still maintains the ability to heterodimerize with VEGFA. (J:271257)

Expression

In Mice Carrying this Mutation:

24 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pgf Mutation: 17 strains or lines available

References

• Original: J:271257 Apicella I, et al., Full Functional Knockout of Placental Growth Factor by Knockin with an Inactive Variant Able to Heterodimerize with VEGF-A. Cell Rep. 2018 Jun 19;23(12):3635-3646
• All: 1 reference(s)