Gt(ROSA)26Sor^{em1(CAG-NGLY1)Lutzy}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{em1(CAG-NGLY1)Lutzy}
• Name: gene trap ROSA 26, Philippe Soriano; endonuclease-mediated mutation 1, Cathy Lutz
• MGI ID: MGI:6280484
• Synonyms: Gt(ROSA)26Sor^{tm1(CAG-LSL-NGLY1)Lutzy}
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{em1(CAG-NGLY1)Lutzy} page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Conditional ready, Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: CRISPR/Cas9 genome editing is used to insert the following construct into the endogenous locus: chicken actin splice donor, rabbit beta-globin splice acceptor, a loxP site, a STOP cassette (3 repeats of SV40 polyA termination signal), a loxP site, and full length human NGLY1, N-glycanase 1, under the control of the CAG promoter. (J:101977)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1089 strains or lines available

References

• Original: J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017
• All: 1 reference(s)