Kiss1^{tm1.1(cre/EGFP)Rpa}
Targeted Allele Detail

Summary

• Symbol: Kiss1^{tm1.1(cre/EGFP)Rpa}
• Name: KiSS-1 metastasis-suppressor; targeted mutation 1.1, Richard D Palmiter
• MGI ID: MGI:6278139
• Synonyms: Kiss1^Cre:GFP, Kiss1^Cre (v2)
• Gene: Kiss1 Location: Chr1:133254950-133257460 bp, + strand Genetic Position: Chr1, 57.91 cM
• Alliance: Kiss1^{tm1.1(cre/EGFP)Rpa} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:270342
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Null/knockout, Recombinase, Reporter)
• Mutations: Insertion, Intragenic deletion
• Kiss1^{tm1.1(cre/EGFP)Rpa} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Kiss1 (MGI:2663985)

• Mutation details: The targeting construct contains a Cre:GFP fusion carrying Myc 3' UTR pA, and an FRT-SvNeo-FRT cassette introduced to the end of the first exon, such that the sequence between the start codon and the end of that exon are deleted. Flp-mediated recombination removed the FRT-flanked neo cassette. The construct removes the nuclear localization signal from cre, uses a non-optimal initiation codon, removes part of the N-terminal sequence of cre, adds a 3' untranslated region from the Myc gene that promotes a short mRNA half-life, and deletes part of exon 1 that includes the signal peptide. (J:270342)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Kiss1 (mouse) Summary of all recombinase alleles driven by Kiss1. Comparative matrix view of recombinase activities Alliance mouse Kiss1 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Kiss1 Mutation: 21 strains or lines available

References

• Original: J:270342 Padilla SL, et al., A Neural Circuit Underlying the Generation of Hot Flushes. Cell Rep. 2018 Jul 10;24(2):271-277
• All: 8 reference(s)