Kdm6b^em1Masn
Endonuclease-mediated Allele Detail

Summary

• Symbol: Kdm6b^em1Masn
• Name: KDM1 lysine (K)-specific demethylase 6B; endonuclease-mediated mutation 1, Masahide Asano
• MGI ID: MGI:6277163
• Synonyms: Jmjd3^deltaDM
• Gene: Kdm6b Location: Chr11:69289334-69304501 bp, - strand Genetic Position: Chr11, 42.6 cM
• Alliance: Kdm6b^em1Masn page

Mutation origin

• Strain of Origin: (C57BL/6 x DBA/2)F1

Mutation description

• Allele Type: Endonuclease-mediated (Not Applicable)
• Mutation: Intragenic deletion
• Mutation details: CRISPR/Cas9 technology generated a 27 bp deletion within exon 18, including the coding sequence for the demethylase activity of Kdm6b. This allele lacks the splice acceptor of exon 18, resulting in two types of mRNA; an r.4570_4599del that encodes a predicted protein that contains a deletion of amino acids 1387-1396 (deltaDM) and is predicted to lose H3K27 demethylase activity and a second mRNA transcribed from exon 17 through intron 17, producing a nonsense mutation in intron 17 which results in a predicted C-terminal deletion (deltaC). qRT-PCR shows that the ratio of deltaDM mRNA to deltaC mRNA is about 1.5 to 1. (J:247259)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Kdm6b Mutation: 65 strains or lines available

References

• Original: J:247259 Naruse C, et al., New insights into the role of Jmjd3 and Utx in axial skeletal formation in mice. FASEB J. 2017 Jun;31(6):2252-2266
• All: 1 reference(s)