Fgl1^{tm1b(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Fgl1^{tm1b(EUCOMM)Hmgu}
• Name: fibrinogen-like protein 1; targeted mutation 1b, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:6276619
• Gene: Fgl1 Location: Chr8:41644471-41668193 bp, - strand Genetic Position: Chr8, 23.89 cM
• Alliance: Fgl1^{tm1b(EUCOMM)Hmgu} page
• IMPC: Fgl1 gene page

Mutation origin

• Mutant Cell Line: HEPD0540_3_F06
• Germline Transmission: Earliest citation of germline transmission: J:269578
• Parent Cell Line: JM8A1.N3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Pgk_P
• Mutation details: The L1L2_Pgk_P cassette was inserted at position 41662443 of Chromosome 8 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by a lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the PGK promoter, an SV40 polyA, a second FRT site, and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 41663288. The critical exon(s) is/are thus flanked by loxP sites. Cre mediated recombination without flp expression created a reporter knockout mouse. ELISA and western blot analyses confirmed the absence of protein in the plasma and liver, respectively, from homozygous mice. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:157065, J:269578)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Fgl1 Mutation: 27 strains or lines available

References

• Original: J:269578 Wang J, et al., Fibrinogen-like Protein 1 Is a Major Immune Inhibitory Ligand of LAG-3. Cell. 2019 Jan 10;176(1-2):334-347.e12
• All: 2 reference(s)