Tln1^em1.1Mos
Endonuclease-mediated Allele Detail

Summary

• Symbol: Tln1^em1.1Mos
• Name: talin 1; endonuclease-mediated mutation 1.1, Markus Moser
• MGI ID: MGI:6276574
• Synonyms: Tln1^3mut
• Gene: Tln1 Location: Chr4:43531513-43562583 bp, - strand Genetic Position: Chr4, 23.05 cM
• Alliance: Tln1^em1.1Mos page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:269782
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Endonuclease-mediated (Not Specified)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details:

  CRISPR-targeting introduced nucleotide substitutions in exon 2 that result in the amino acid substitutions of lysine 15 with alanine (p.K15A) and arginine 30 and 35 with alanine (p.R30A, p.R35A). Cre-mediated recombination removed the loxP site flanked neomycin resistance gene cassette inserted into intron 2. (J:269782)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tln1 Mutation: 145 strains or lines available

References

• Original: J:269782 Bromberger T, et al., Direct Rap1/Talin1 interaction regulates platelet and neutrophil integrin activity in mice. Blood. 2018 Dec 27;132(26):2754-2762
• All: 1 reference(s)