Kif6^{tm1b(KOMP)Mbp}
Targeted Allele Detail

Summary

• Symbol: Kif6^{tm1b(KOMP)Mbp}
• Name: kinesin family member 6; targeted mutation 1b, Mouse Biology Program, UC Davis
• MGI ID: MGI:6276220
• Synonyms: Kif6-LacZ^tm1b
• Gene: Kif6 Location: Chr17:49922164-50216875 bp, + strand Genetic Position: Chr17, 25.78 cM, cytoband C
• Alliance: Kif6^{tm1b(KOMP)Mbp} page
• IMPC: Kif6 gene page

Mutation origin

• Mutant Cell Line: EPD0736_3_G01
• Germline Transmission: Earliest citation of germline transmission: J:269442
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 49977578 of Chromosome 17 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 49978471. The critical exon(s) is/are thus flanked by loxP sites. A null/knockout allele was created by cre recombinase expression in mice carrying the tm1a allele to remove the neo selection cassette and loxP-flanked critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:269442)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Kif6 Mutation: 36 strains or lines available

References

• Original: J:269442 Konjikusic MJ, et al., Mutations in Kinesin family member 6 reveal specific role in ependymal cell ciliogenesis and human neurological development. PLoS Genet. 2018 Nov;14(11):e1007817
• All: 1 reference(s)