Amotl1^em2Rstot
Endonuclease-mediated Allele Detail

Summary

• Symbol: Amotl1^em2Rstot
• Name: angiomotin-like 1; endonuclease-mediated mutation 2, Rolf Stottmann
• MGI ID: MGI:6276196
• Synonyms: Amotl1^D1
• Gene: Amotl1 Location: Chr9:14453262-14556352 bp, - strand Genetic Position: Chr9, 4.12 cM, cytoband A3
• Alliance: Amotl1^em2Rstot page

Mutation origin

• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details:

  Exon 3 was targeted using an sgRNA and an ssODN template with CRISPR/Cas9 technology to change arginine (TCG) codon 157 to cysteine (TCT) (p.R157C) and to introduce several silent mutation. An untargeted one-bp deletion was found in this allele, deleting the last residue in serine codon 155 (TCG) resulting in a frameshift and a premature stop codon. (J:270029)

Expression

In Mice Carrying this Mutation:

2 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Amotl1 Mutation: 54 strains or lines available

References

• Original: J:270029 Liegel RP, et al., Using human sequencing to guide craniofacial research. Genesis. 2019 Jan;57(1):e23259
• All: 1 reference(s)