Tg(Myh6-Kcnj11*)1Wco
Transgene Detail

Summary

• Symbol: Tg(Myh6-Kcnj11*)1Wco
• Name: transgene insertion 1, William A Coetzee
• MGI ID: MGI:6275012
• Synonyms: alpha-MHC-Kir6.2-AAA-FLAG, Kir6.2-AAA
• Transgene: Tg(Myh6-Kcnj11*)1Wco Location: unknown
• Alliance: Tg(Myh6-Kcnj11*)1Wco page

Transgene origin

• Strain of Origin: FVB/N

Transgene description

• Transgene Type: Transgenic (Dominant negative, Inserted expressed sequence)
• Mutation: Insertion
• Tg(Myh6-Kcnj11*)1Wco expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Kcnj11 (MGI:107501)	the subunit pore region is mutated to three alanines resulting in a dominant negative

• Mutation details: The alpha-myosin heavy chain promoter drives cardiac-specific expression of a dominant negative mouse Kcnj11 mutant, in which the subunit pore region was mutated from glycine-phenylalanine-glycine to three alanines, with a FLAG tag. RT-PCR and Western blotting confirmed expression of the transgene specifically in the heart. Several lines were made but only one was further analyzed. (J:116284)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:116284 Tong X, et al., Consequences of cardiac myocyte-specific ablation of KATP channels in transgenic mice expressing dominant negative Kir6 subunits. Am J Physiol Heart Circ Physiol. 2006 Aug;291(2):H543-51
• All: 1 reference(s)